The present invention relates to a composition and a kit for regeneration and treatment of cartilage^ preferably^ fibrous cartilage or elastic cartilage and a regeneration method using the same. A composition and a kit for cartilage regeneration according to the present invention can be administered simply in a minimally invasive manner to a site in which fibrous cartilage or elastic cartilage is needed to be regenerated or restored from injury^ exhibit resistance to degradation enzymes without toxicity within the body^ and are attached to or detained at and thus retained at the injured^ administered site^ whereby behavioral improvement can be brought about in surrounding cells^ which leads to effectively inducing defected tissues of the meniscus to be regenerated. Therefore^ the composition of the present invention is useful as a mediator for aiding the regeneration of biological tissue defected regions in the biomaterials field.