METHOD FOR PREPARING CARBOHYDRATE CONVERTING ENZYME LIBRARY USING DOMAIN SWAPPING BASED ON AMINO ACID CONSERVED SEQUENCE AND USE THEREOF
METHOD FOR PREPARING CARBOHYDRATE CONVERTING ENZYME LIBRARY USING DOMAIN SWAPPING BASED ON AMINO ACID CONSERVED SEQUENCE AND USE THEREOF
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작성자 관리자 작성일 조회648회관련링크
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해외/PCT 출원 : 2016.08.01
해외/PCT 등록일자 :
해외/PCT 등록번호 : PCTKR2016008463
The present invention relates to a method for preparing a chimeric carbohydrate converting enzyme library, the chimeric carbohydrate converting enzyme library prepared through the method and the carbohydrate converting enzyme prepared through the library, wherein the method comprises identifying a conserved sequence of a carbohydrate converting enzyme and recombining the conserved sequence. By using the smart carbohydrate converting enzyme library according to the present invention, the effect can be realized in that a carbohydrate converting enzyme can be obtained in which the activity and function of the enzyme is not lost at an efficiency of 40% or more, unlike a typical library, and a carbohydrate converting enzyme having various physical and biochemical properties is obtainable. Therefore, an enzyme suitable for various conditions can be selected and used.
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