The present invention relates to a mass production method of phenylacetyl homoserine lactones. The production method of phenylacetyl homoserine lactones of the present invention significantly increases the production of phenylacetyl homoserine lactone derivatives by using an E. coli expression system with an advantage of quick, high concentration culturing in an inexpensive medium being possible, and an in vitro enzymatic reaction and a bioconversion system, and thus can be useful in the mass production of phenylacetyl homoserine lactone derivatives.